What bad day?
Good news. I got out of work earlier than I was thinking I'd would.
Bad news. It's because I had to throw seven hours of bench work in the autoclave bag.
Boss decided that it wasn't worth risking clogging the sexy expensive sorter. Especially since I know that my cell count is way off. He was trying to tell me it's the media killing crap.
"Uhh, no. This has been a problem since December. We've even used a new lot of FBS since then."
"We have a lot of data that you shouldn't be getting that crap."
"From five days?"
"Yes."
I tried hard not to sneeze "bulls**t". I'll go back, but there's no useful lymphoycte data that you get if you're trying to study MATURE lymphocytes at 5 days. I'm not looking for mature lymphocytes, so that's okay.
But he did give me permission to treat with NH4Cl
Bad news. It's because I had to throw seven hours of bench work in the autoclave bag.
Boss decided that it wasn't worth risking clogging the sexy expensive sorter. Especially since I know that my cell count is way off. He was trying to tell me it's the media killing crap.
"Uhh, no. This has been a problem since December. We've even used a new lot of FBS since then."
"We have a lot of data that you shouldn't be getting that crap."
"From five days?"
"Yes."
I tried hard not to sneeze "bulls**t". I'll go back, but there's no useful lymphoycte data that you get if you're trying to study MATURE lymphocytes at 5 days. I'm not looking for mature lymphocytes, so that's okay.
But he did give me permission to treat with NH4Cl
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